Skip to main content
Addgene

mCherry-eDHFR
(Plasmid #107266)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 107266 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pIVT
  • Backbone manufacturer
    Carmen Williams (Addgene plasmid #32374)
  • Total vector size (bp) 4850
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    mCherry-eDHFR
  • Species
    E.coli
  • Insert Size (bp)
    1218
  • Entrez Gene
    dhfr (a.k.a. D616_p72001)
  • Promoter T7
  • Tag / Fusion Protein
    • mCherry (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site SacI (not destroyed)
  • 5′ sequencing primer T7 promotor
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    mCherry-eDHFR was a gift from Michael Lampson (Addgene plasmid # 107266 ; http://n2t.net/addgene:107266 ; RRID:Addgene_107266)

  • For your References section:

    Spindle asymmetry drives non-Mendelian chromosome segregation. Akera T, Chmatal L, Trimm E, Yang K, Aonbangkhen C, Chenoweth DM, Janke C, Schultz RM, Lampson MA. Science. 2017 Nov 3;358(6363):668-672. doi: 10.1126/science.aan0092. 10.1126/science.aan0092 PubMed 29097549
Related items:
Commonly requested with: