6x Halo-EGFP- 2x PACT
(Plasmid
#107265)
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Purpose6x Halo-EGFP- 2x PACT construct downstream of T7 promotor for in vitro transcription
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 107265 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepERB219
- Total vector size (bp) 15189
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert name6x Halo-EGFP- 2x PACT
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SpeciesH. sapiens (human)
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Insert Size (bp)7334
- Promoter T7
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Tag
/ Fusion Protein
- GFP
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer T7 promotor (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
6x Halo-EGFP- 2x PACT was a gift from Michael Lampson (Addgene plasmid # 107265 ; http://n2t.net/addgene:107265 ; RRID:Addgene_107265) -
For your References section:
Spindle asymmetry drives non-Mendelian chromosome segregation. Akera T, Chmatal L, Trimm E, Yang K, Aonbangkhen C, Chenoweth DM, Janke C, Schultz RM, Lampson MA. Science. 2017 Nov 3;358(6363):668-672. doi: 10.1126/science.aan0092. 10.1126/science.aan0092 PubMed 29097549
