pAGH51
(Plasmid #107255)

• Purpose: HU-FRB construct (anchor) expressed in trans from the slr0168 neutral site of Synechocystis PCC6803. Used to anchor away any FKBP tagged target in presence of rapamycin.
• Depositing Lab: Erin O'Shea
• Publication: Gutu et al Mol Microbiol. 2018 Jan 22. doi: 10.1111/mmi.13912.

Backbone

• Vector backbone: pBR322
• Backbone size w/o insert (bp): 4313
• Total vector size (bp): 8600
• Modifications to backbone: used gibson cloning
• Vector type: Synthetic Biology

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: HU-FRB
• Alt name: sll1712 of Synechocystis sp. PCC6803
• Alt name: histone-like DNA binding protein
• Species: Synthetic; Synecocystis sp. PCC6803
• Insert Size (bp): 4324
• Promoter: native HU promoter
• Tag / Fusion Protein:
  • FRB (C terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: TCTTGTGCAATGTAACATCAGAG
• 3′ sequencing primer: GTCATGATCTTCGATGTAAGTCAGG

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry