pAGH50
(Plasmid #107254)

• Purpose: Vipp1-GFP-FKBP12 construct (spec cassette downstream) used to anchor away Vipp1 (sll0617) of Synecocystis PCC6803.
• Depositing Lab: Erin O'Shea
• Publication: Gutu et al Mol Microbiol. 2018 Jan 22. doi: 10.1111/mmi.13912.

Backbone

• Vector backbone: pGEM-3ZfP
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 3170
• Total vector size (bp): 8170
• Modifications to backbone: cloned the insert by Gibson into BamHI/SphI linearized vector backbone
• Vector type: Bacterial Expression ; suicide vector for destined for double homologous recombination of the insert

Growth in Bacteria

• Bacterial Resistance(s): Spectinomycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: vipp1-gfp-fkbp12
• Species: Synthetic; Synechocystis sp. PCC6803
• Insert Size (bp): 5330
• Promoter: native
• Tag / Fusion Protein:
  • GFP and FKBP12 (N terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: t7 forward
• 3′ sequencing primer: M13 reverse

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

the construct is similar to pAGH42 except that FKBP12 encoding fragment was fused in frame with Vipp1-GFP.