pUC57-PP7v3
(Plasmid
#107230)
-
PurposeExpresses 24x optimized PP7 stem loops (version 3) in bacterial cells
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 107230 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepUC57
- Total vector size (bp) 4320
-
Vector typeBacterial Expression
Growth in Bacteria
-
Bacterial Resistance(s)Kanamycin, 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert name24xPP7v3
-
SpeciesSynthetic
-
Insert Size (bp)1584
-
Tags
/ Fusion Proteins
- P2A site (N terminal on insert)
- 3x FLAG-tag (C terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer M13-fwd (Common Sequencing Primers)
Resource Information
-
A portion of this plasmid was derived from a plasmid made byThe gene was synthesized by Genewiz.
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pUC57-PP7v3 was a gift from Jeffrey Chao (Addgene plasmid # 107230 ; http://n2t.net/addgene:107230 ; RRID:Addgene_107230) -
For your References section:
Single-Molecule Quantification of Translation-Dependent Association of mRNAs with the Endoplasmic Reticulum. Voigt F, Zhang H, Cui XA, Triebold D, Liu AX, Eglinger J, Lee ES, Chao JA, Palazzo AF. Cell Rep. 2017 Dec 26;21(13):3740-3753. doi: 10.1016/j.celrep.2017.12.008. 10.1016/j.celrep.2017.12.008 PubMed 29281824