Skip to main content
Addgene

MSGV1-1D3-28Z.1-3 mut
(Plasmid #107227)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 107227 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    MSGV1
  • Backbone size w/o insert (bp) 5586
  • Vector type
    Retroviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    1D3-28Z. 1-3 mut
  • Alt name
    Anti-mouse CD19 CAR with CD3 ITAMs mutated
  • Species
    Synthetic
  • Insert Size (bp)
    1427
  • Mutation
    None
  • GenBank ID
    None
  • Tag / Fusion Protein
    • None

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (unknown if destroyed)
  • 3′ cloning site SalI (unknown if destroyed)
  • 5′ sequencing primer unknown
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MSGV1-1D3-28Z.1-3 mut was a gift from James Kochenderfer & Steven Rosenberg (Addgene plasmid # 107227 ; http://n2t.net/addgene:107227 ; RRID:Addgene_107227)

  • For your References section:

    Adoptive transfer of syngeneic T cells transduced with a chimeric antigen receptor that recognizes murine CD19 can eradicate lymphoma and normal B cells. Kochenderfer JN, Yu Z, Frasheri D, Restifo NP, Rosenberg SA. Blood. 2010 Nov 11;116(19):3875-86. doi: 10.1182/blood-2010-01-265041. Epub 2010 Jul 14. 10.1182/blood-2010-01-265041 PubMed 20631379
Related items:
Commonly requested with: