GW1-mito-roGFP1-mApple
(Plasmid
#107218)
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PurposeMammalian expression plasmid to target the roGFP1-mApple sensor to the mitochondrial matrix.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 107218 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneGW1-mito
- Backbone size w/o insert (bp) 5649
- Total vector size (bp) 7137
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameroGFP1-mApple
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Insert Size (bp)1488
- Promoter CMV
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Tag
/ Fusion Protein
- 4xCox8 (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site SgrAI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer CTG AAT CGG AGT GAG TGC
- 3′ sequencing primer CCT CTA CAA ATG TGG TAT GG (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
GW1-mito-roGFP1-mApple was a gift from Mathew Tantama (Addgene plasmid # 107218 ; http://n2t.net/addgene:107218 ; RRID:Addgene_107218) -
For your References section:
Extending roGFP Emission via Forster-Type Resonance Energy Transfer Relay Enables Simultaneous Dual Compartment Ratiometric Redox Imaging in Live Cells. Norcross S, Trull KJ, Snaider J, Doan S, Tat K, Huang L, Tantama M. ACS Sens. 2017 Nov 22;2(11):1721-1729. doi: 10.1021/acssensors.7b00689. Epub 2017 Nov 7. 10.1021/acssensors.7b00689 PubMed 29072071
Map uploaded by the depositor.
