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Addgene

xyl8.3
(Plasmid #107217)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 107217 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pETMBPH
  • Total vector size (bp) 6263
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    xyl8.3
  • Species
    Synthetic
  • Insert Size (bp)
    1002

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (unknown if destroyed)
  • 3′ cloning site PstI (unknown if destroyed)
  • 5′ sequencing primer ACTAGTGGTTCTGGTTCCGCGGGTG
  • 3′ sequencing primer CCCGTTTAGAGGCCCCAAGG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    xyl8.3 was a gift from Sarel Fleishman (Addgene plasmid # 107217 ; http://n2t.net/addgene:107217 ; RRID:Addgene_107217)

  • For your References section:

    Highly active enzymes by automated combinatorial backbone assembly and sequence design. Lapidoth G, Khersonsky O, Lipsh R, Dym O, Albeck S, Rogotner S, Fleishman SJ. Nat Commun. 2018 Jul 17;9(1):2780. doi: 10.1038/s41467-018-05205-5. 10.1038/s41467-018-05205-5 PubMed 30018322
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