ecAT3.10
(Plasmid #107215)

• Purpose: Mammalian expression plasmid to target the ecAT3.10 extracellular ATP sensor to the cell surface.
• Depositing Lab: Mathew Tantama
• Publication: Conley et al PLoS One. 2017 Nov 9;12(11):e0187481. doi: 10.1371/journal.pone.0187481. eCollection 2017.

Backbone

• Vector backbone: pCMV(MinDis) [variant of pDisplay]
• Backbone manufacturer: modified from Invitrogen
• Backbone size w/o insert (bp): 5000
• Total vector size (bp): 7074
• Modifications to backbone: From pDisplay: Hemagglutinin (HA) tag removed
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: ecAT3.10
• Species: Synthetic
• Insert Size (bp): 2070
• Promoter: CMV

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: CMV Forward
• 3′ sequencing primer: BGHR

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry