GFP-S100A11
(Plasmid
#107201)
-
PurposeExpresses GFP-human S100A11 fusion protein for live cell imaging
-
Depositing Labs
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 107201 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepEGFP-C2
-
Backbone manufacturerClontech
- Backbone size w/o insert (bp) 4700
- Total vector size (bp) 5000
-
Vector typeMammalian Expression
-
Selectable markersNeomycin (select with G418)
Growth in Bacteria
-
Bacterial Resistance(s)Kanamycin, 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberUnknown
Gene/Insert
-
Gene/Insert nameS100A11
-
Alt nameS100C
-
Alt nameCalgizzarin
-
SpeciesH. sapiens (human)
-
Insert Size (bp)318
-
GenBank IDNM_005620.1
-
Entrez GeneS100A11 (a.k.a. HEL-S-43, MLN70, S100C)
- Promoter CMV
-
Tag
/ Fusion Protein
- Green Fluorescent Protein (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site PstI (not destroyed)
- 5′ sequencing primer EGFP-C (Common Sequencing Primers)
Resource Information
-
Article Citing this Plasmid
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
GFP-S100A11 was a gift from Volker Gerke & Ursula Rescher (Addgene plasmid # 107201 ; http://n2t.net/addgene:107201 ; RRID:Addgene_107201)