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Addgene

YFP-S100A10
(Plasmid #107200)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 107200 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEYFP-C1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4700
  • Total vector size (bp) 5000
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    S100A10
  • Alt name
    p11
  • Alt name
    annexin A2 light chain
  • Alt name
    Calpactin light chain
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    294
  • GenBank ID
    M_002966.2
  • Entrez Gene
    S100A10 (a.k.a. 42C, ANX2L, ANX2LG, CAL1L, CLP11, Ca[1], GP11, P11, p10)
  • Promoter CMV
  • Tag / Fusion Protein
    • Yellow Fluorescent Protein (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer EGFP-C
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    YFP-S100A10 was a gift from Volker Gerke & Ursula Rescher (Addgene plasmid # 107200 ; http://n2t.net/addgene:107200 ; RRID:Addgene_107200)

  • For your References section:

    Cell-surface attachment of pedestal-forming enteropathogenic E. coli induces a clustering of raft components and a recruitment of annexin 2. Zobiack N, Rescher U, Laarmann S, Michgehl S, Schmidt MA, Gerke V. J Cell Sci. 2002 Jan 1;115(Pt 1):91-8. PubMed 11801727
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