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Addgene

anxA10-GFP
(Plasmid #107199)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 107199 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP-N3
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4700
  • Total vector size (bp) 5700
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    annexin A10
  • Alt name
    ANX14
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    975
  • Entrez Gene
    ANXA10 (a.k.a. ANX14)
  • Promoter CMV
  • Tag / Fusion Protein
    • Green Fluorescent Protein (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer EGFP-N
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    anxA10-GFP was a gift from Volker Gerke & Ursula Rescher (Addgene plasmid # 107199 ; http://n2t.net/addgene:107199 ; RRID:Addgene_107199)

  • For your References section:

    The tumor suppressor annexin A10 is a novel component of nuclear paraspeckles. Quiskamp N, Poeter M, Raabe CA, Hohenester UM, Konig S, Gerke V, Rescher U. Cell Mol Life Sci. 2014 Jan;71(2):311-29. doi: 10.1007/s00018-013-1375-4. Epub 2013 May 29. 10.1007/s00018-013-1375-4 PubMed 23715859
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