anxA9-GFP
(Plasmid
#107198)
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PurposeExpresses human annexin A9-GFP fusion protein for live cell imaging
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Depositing Labs
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 107198 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepEGFP-N3
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Backbone manufacturerClontech
- Backbone size w/o insert (bp) 4700
- Total vector size (bp) 5700
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameannexin A9
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Alt nameANX31
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SpeciesH. sapiens (human)
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Insert Size (bp)1035
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Entrez GeneANXA9 (a.k.a. ANX31)
- Promoter CMV
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Tag
/ Fusion Protein
- Green Fluorescent Protein (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site SalI (not destroyed)
- 5′ sequencing primer EGFP-N (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
anxA9-GFP was a gift from Volker Gerke & Ursula Rescher (Addgene plasmid # 107198 ; http://n2t.net/addgene:107198 ; RRID:Addgene_107198) -
For your References section:
Atypical properties displayed by annexin A9, a novel member of the annexin family of Ca(2+) and lipid binding proteins. Goebeler V, Ruhe D, Gerke V, Rescher U. FEBS Lett. 2003 Jul 10;546(2-3):359-64. S0014579303006343 [pii] PubMed 12832069
