cmamxA2-GFP
(Plasmid #107197)

• Purpose: Expresses human annexin A2-GFP fusion protein for live cell imaging, all type II Ca2+ binding sites deleted
• Depositing Labs: Volker Gerke, Ursula Rescher
• Publication: Rescher et al J Cell Sci. 2000 Nov;113 ( Pt 22):3931-8.

Backbone

• Vector backbone: pEGFP-N3
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4700
• Total vector size (bp): 5700
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: cmannexin A2
• Alt name: Calpactin heavy chain
• Alt name: cmanxA2
• Species: H. sapiens (human)
• Insert Size (bp): 1023
• Mutation: all type II Ca2+ binding sites deleted ( D161A, E246A, D321A); Ala residue 65 in human sequence replaced by Glu found at the corresponding position in the porcine sequence, allowing specific detection by monoclonal anti-AnxA2 antibody H28 (PMID: 3126079).
• GenBank ID: NM_001002857.1
• Entrez Gene: ANXA2 (a.k.a. ANX2, ANX2L4, CAL1H, HEL-S-270, LIP2, LPC2, LPC2D, P36, PAP-IV)
• Promoter: CMV
• Tag / Fusion Protein:
  • Green Fluorescent Protein (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BglII (not destroyed)
• 3′ cloning site: SalI (not destroyed)
• 5′ sequencing primer: EGFP-N

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry