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Addgene

cmamxA1-GFP
(Plasmid #107195)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 107195 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP-N3
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4700
  • Total vector size (bp) 5800
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    cmannexin A1
  • Species
    Sus scrofa
  • Insert Size (bp)
    1134
  • Mutation
    exchanges Asp170Ala, Glu255Ala, Glu330Ala, all type II Ca2+ binding sites deleted
  • GenBank ID
    NM_001163998.1
  • Promoter CMV
  • Tag / Fusion Protein
    • Green Fluorescent Protein (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer EGFP-N
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    cmamxA1-GFP was a gift from Volker Gerke & Ursula Rescher (Addgene plasmid # 107195 ; http://n2t.net/addgene:107195 ; RRID:Addgene_107195)

  • For your References section:

    Intact Ca(2+)-binding sites are required for targeting of annexin 1 to endosomal membranes in living HeLa cells. Rescher U, Zobiack N, Gerke V. J Cell Sci. 2000 Nov;113 ( Pt 22):3931-8. PubMed 11058080
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