1247 pCMVb p300 HA delta33
(Plasmid #10719)

• Depositing Lab: William Sellers
• Publication: p300 (unpublished)

Backbone

• Vector backbone: CMVbeta
• Backbone manufacturer: not the same as the commercial CMVb
• Backbone size w/o insert (bp): 4800
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: p300 delta33
• Alt name: p300
• Species: H. sapiens (human)
• Insert Size (bp): 7700
• Mutation: Mutant does not bind to E1A. Amino acids 1737 to 1836 deleted.
• GenBank ID: U01877
• Entrez Gene: EP300 (a.k.a. KAT3B, MKHK2, RSTS2, p300)
• Tag / Fusion Protein:
  • HA (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NotI (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: CMV Forward
• 3′ sequencing primer: M13 reverse

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

see author's map for pCMVb p300 wt for map of empty pCMVb vector.