pAAV-CAG-EGFPKir7.1
(Plasmid
#107182)
-
PurposeAdenoassociatedvirus particle generation
-
Depositing Lab
-
Publication
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 107182 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepAV[Exp]-Bsd
-
Backbone manufacturerVector builder
-
Vector typeAAV
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)NEB Stable
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameKCNJ13
-
SpeciesH. sapiens (human)
-
GenBank IDNM_002242.4
-
Entrez GeneKCNJ13 (a.k.a. KIR1.4, KIR7.1, LCA16, SVD)
- Promoter CAG
-
Tag
/ Fusion Protein
- EGFP (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
- 5′ sequencing primer CTATGACCATGATTACGAATTGCC
- 3′ sequencing primer CCTGAAACATAAAATGAATGCAATTG (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pAAV-CAG-EGFPKir7.1 was a gift from Bikash Pattnaik (Addgene plasmid # 107182 ; http://n2t.net/addgene:107182 ; RRID:Addgene_107182)