hACTB-RFP-Kir7.1
(Plasmid
#107179)
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PurposeExpression of N-terminal fused Kir7.1 in mammalian cells.
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 107179 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepRP[Exp]-Bsd
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Backbone manufacturerVector Builder
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameKCNJ13
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SpeciesH. sapiens (human)
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GenBank IDNM_002242.4
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Entrez GeneKCNJ13 (a.k.a. KIR1.4, KIR7.1, LCA16, SVD)
- Promoter hACTB
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Tag
/ Fusion Protein
- RFP (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
- 5′ sequencing primer NA (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
hACTB-RFP-Kir7.1 was a gift from Bikash Pattnaik (Addgene plasmid # 107179 ; http://n2t.net/addgene:107179 ; RRID:Addgene_107179)
