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pGEX-6P-3-F37A+L196F+V252A+C253G-hADPRM
(Plasmid #107163)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 107163 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEX-6P-3
  • Backbone manufacturer
    GE Healthcare
  • Backbone size w/o insert (bp) 4946
  • Total vector size (bp) 6008
  • Modifications to backbone
    None
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    F37A+L196F+V252A+C253G-hADPRM GST-mutant cADPR phosphohydrolase gene
  • Species
    Synthetic
  • Insert Size (bp)
    1062
  • GenBank ID
    MF447900.1
  • Promoter tac
  • Tag / Fusion Protein
    • GST (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SalI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer pGEX Fwd
  • 3′ sequencing primer pGEX Rev
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEX-6P-3-F37A+L196F+V252A+C253G-hADPRM was a gift from Jose Carlos Cameselle (Addgene plasmid # 107163 ; http://n2t.net/addgene:107163 ; RRID:Addgene_107163)

  • For your References section:

    Specific cyclic ADP-ribose phosphohydrolase obtained by mutagenic engineering of Mn(2+)-dependent ADP-ribose/CDP-alcohol diphosphatase. Ribeiro JM, Canales J, Cabezas A, Rodrigues JR, Pinto RM, Lopez-Villamizar I, Costas MJ, Cameselle JC. Sci Rep. 2018 Jan 18;8(1):1036. doi: 10.1038/s41598-017-18393-9. 10.1038/s41598-017-18393-9 [pii] PubMed 29348648
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