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pBHt2G-RFP
(Plasmid #107162)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 107162 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBHt2G
  • Backbone manufacturer
    Khang et al, 2010 http://www.plantcell.org/content/22/4/1388
  • Backbone size (bp) 9617
  • Modifications to backbone
    Removal of BsaI sites to domesticate backbone for Golden Gate (GG) cloning. Addition of an RFP marker for red-white selection in E. coli. The RFP is flanked by two BsaI sites. GG reaction leads to the loss of the RFP when the gene is inserted. The RFP is expressed in E. coli, allowing for red-white selection of transformants. Antibiotic resistance changed to Ampicillin
  • Vector type
    Fungal grobacterium transformation plasmid, tested in Magnaporthe
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Golden-Gate compatible Magnaporthe oryzae Agrobacterium transformation vectors. Pennington HG, Youles M, Kamoun S. Figshare (2018) 10.6084/m9.figshare.5808348

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBHt2G-RFP was a gift from Sophien Kamoun (Addgene plasmid # 107162 ; http://n2t.net/addgene:107162 ; RRID:Addgene_107162)
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