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MBP-rPKL S12TAG
(Plasmid #107155)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 107155 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    PCRT7
  • Backbone size w/o insert (bp) 4467
  • Total vector size (bp) 7277
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MBP-tagged rat pyruvate kinase liver isoform
  • Alt name
    rPKL
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    2810
  • Mutation
    Serine 12 to TAG for phosphoserine insertion
  • Entrez Gene
    Pklr (a.k.a. PK1, PKL, Pklg)
  • Promoter PBAD
  • Tags / Fusion Proteins
    • C-term 6-his
    • N-term MBP

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site SacI (not destroyed)
  • 5′ sequencing primer CCTACCTGACGCTTTTTATCGCAACTC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MBP-rPKL S12TAG was a gift from Jesse Rinehart (Addgene plasmid # 107155 ; http://n2t.net/addgene:107155 ; RRID:Addgene_107155)

  • For your References section:

    Distinct Hepatic PKA and CDK Signaling Pathways Control Activity-Independent Pyruvate Kinase Phosphorylation and Hepatic Glucose Production. Gassaway BM, Cardone RL, Padyana AK, Petersen MC, Judd ET, Hayes S, Tong S, Barber KW, Apostolidi M, Abulizi A, Sheetz JB, Kshitiz, Aerni HR, Gross S, Kung C, Samuel VT, Shulman GI, Kibbey RG, Rinehart J. Cell Rep. 2019 Dec 10;29(11):3394-3404.e9. doi: 10.1016/j.celrep.2019.11.009. 10.1016/j.celrep.2019.11.009 PubMed 31825824
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