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pGEX-6P1-Vinculin tail
(Plasmid #107154)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 107154 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEX-6P1
  • Backbone manufacturer
    GE Life Sciences
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Vinculin
  • Alt name
    VCL
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    528
  • Entrez Gene
    VCL (a.k.a. CMD1W, CMH15, HEL114, MV, MVCL, VINC)
  • Promoter Ptac
  • Tag / Fusion Protein
    • GST (N terminal on backbone)

Cloning Information

  • Cloning method Gibson Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

GST tag in frame with vinculin tail

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEX-6P1-Vinculin tail was a gift from Tina Izard (Addgene plasmid # 107154 ; http://n2t.net/addgene:107154 ; RRID:Addgene_107154)

  • For your References section:

    Lipid binding promotes oligomerization and focal adhesion activity of vinculin. Chinthalapudi K, Rangarajan ES, Patil DN, George EM, Brown DT, Izard T. J Cell Biol. 2014 Dec 8;207(5):643-56. doi: 10.1083/jcb.201404128. 10.1083/jcb.201404128 PubMed 25488920
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