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pcDNA4-GFP-Vinculin
(Plasmid #107153)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 107153 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCDNA4
  • Backbone manufacturer
    Thermo Fisher Scientific
  • Vector type
    Mammalian Expression
  • Selectable markers
    Zeocin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Vinculin
  • Alt name
    VCL
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3201
  • Entrez Gene
    VCL (a.k.a. CMD1W, CMH15, HEL114, MV, MVCL, VINC)
  • Promoter CMV
  • Tag / Fusion Protein
    • (His)10-GFP (N terminal on backbone)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

GFP tag in frame with metavinculin gene

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA4-GFP-Vinculin was a gift from Tina Izard (Addgene plasmid # 107153 ; http://n2t.net/addgene:107153 ; RRID:Addgene_107153)

  • For your References section:

    Lipid binding promotes oligomerization and focal adhesion activity of vinculin. Chinthalapudi K, Rangarajan ES, Patil DN, George EM, Brown DT, Izard T. J Cell Biol. 2014 Dec 8;207(5):643-56. doi: 10.1083/jcb.201404128. 10.1083/jcb.201404128 PubMed 25488920
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