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pET28-Metavinculin tail
(Plasmid #107152)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 107152 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET28
  • Backbone manufacturer
    Novagen (EMD Millipore)
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    metavinculin
  • Alt name
    VCL
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    531
  • Entrez Gene
    VCL (a.k.a. CMD1W, CMH15, HEL114, MV, MVCL, VINC)
  • Promoter T7
  • Tag / Fusion Protein
    • (His)8 (N terminal on backbone)

Cloning Information

  • Cloning method Gibson Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Octahistidine and a prescission protease site in frame with metavinculin tail

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28-Metavinculin tail was a gift from Tina Izard (Addgene plasmid # 107152 ; http://n2t.net/addgene:107152 ; RRID:Addgene_107152)

  • For your References section:

    Differential lipid binding of vinculin isoforms promotes quasi-equivalent dimerization. Chinthalapudi K, Rangarajan ES, Brown DT, Izard T. Proc Natl Acad Sci U S A. 2016 Aug 23;113(34):9539-44. doi: 10.1073/pnas.1600702113. Epub 2016 Aug 8. 10.1073/pnas.1600702113 PubMed 27503891
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