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Purposemammalian expression
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 107150 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCDNA3
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Backbone manufacturerThermo Fisher Scientific
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameneurofibromin 2
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Alt nameNF2
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Alt nameSCH
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SpeciesH. sapiens (human)
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Insert Size (bp)1785
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Entrez GeneNF2 (a.k.a. ACN, BANF, SCH, SWNV, merlin-1)
- Promoter CMV
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Tag
/ Fusion Protein
- Flag (N terminal on backbone)
Cloning Information
- Cloning method Gibson Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Flag tag in frame with hNF2 gene
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3-Flag-hNF2 was a gift from Tina Izard (Addgene plasmid # 107150 ; http://n2t.net/addgene:107150 ; RRID:Addgene_107150) -
For your References section:
Lipid binding promotes the open conformation and tumor-suppressive activity of neurofibromin 2. Chinthalapudi K, Mandati V, Zheng J, Sharff AJ, Bricogne G, Griffin PR, Kissil J, Izard T. Nat Commun. 2018 Apr 6;9(1):1338. doi: 10.1038/s41467-018-03648-4. 10.1038/s41467-018-03648-4 PubMed 29626191