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pET32a-Trx-PP-hNF2
(Plasmid #107148)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 107148 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET32a
  • Backbone manufacturer
    Novagen (EMD Millipore)
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    neurofibromin 2
  • Alt name
    NF2
  • Alt name
    SCH
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1785
  • Entrez Gene
    NF2 (a.k.a. ACN, BANF, SCH, SWNV, merlin-1)
  • Promoter T7
  • Tag / Fusion Protein
    • (His)6-Thioredoxin (N terminal on backbone)

Cloning Information

  • Cloning method Gibson Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Prescission protease site is inserted in frame with NF2 gene

Note: The NFS insert contains A190V and G302E mutations compared to the closest reference sequence (NP_000259.1). The depositor has confirmed that these do not affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET32a-Trx-PP-hNF2 was a gift from Tina Izard (Addgene plasmid # 107148 ; http://n2t.net/addgene:107148 ; RRID:Addgene_107148)

  • For your References section:

    Lipid binding promotes the open conformation and tumor-suppressive activity of neurofibromin 2. Chinthalapudi K, Mandati V, Zheng J, Sharff AJ, Bricogne G, Griffin PR, Kissil J, Izard T. Nat Commun. 2018 Apr 6;9(1):1338. doi: 10.1038/s41467-018-03648-4. 10.1038/s41467-018-03648-4 PubMed 29626191
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