MSCV-mouse p19ARF-IRES-GFP
(Plasmid
#107147)
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PurposeMammalian expression of mouse Arf
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 107147 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneMSCV-IRES-GFP
- Backbone size w/o insert (bp) 6573
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Vector typeMammalian Expression, Retroviral
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Selectable markersGFP
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameArf [alternative reading frame]
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SpeciesM. musculus (mouse)
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Entrez GeneCdkn2a (a.k.a. ARF-INK4a, Arf, INK4a-ARF, Ink4a/Arf, MTS1, Pctr1, p16, p16(INK4a), p16INK4a, p19<ARF>, p19ARF)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer unknown (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byp19Arf was originally cloned by Dr. Charles J. Sherr, St. Jude Children's Research Hospital
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
MSCV-mouse p19ARF-IRES-GFP was a gift from Martine Roussel (Addgene plasmid # 107147 ; http://n2t.net/addgene:107147 ; RRID:Addgene_107147) -
For your References section:
Arf induces p53-dependent and -independent antiproliferative genes. Kuo ML, Duncavage EJ, Mathew R, den Besten W, Pei D, Naeve D, Yamamoto T, Cheng C, Sherr CJ, Roussel MF. Cancer Res. 2003 Mar 1;63(5):1046-53. PubMed 12615721