pBABE-SSeCKs T751A
(Plasmid
#107142)
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Purposefull-length murine SSeCKs/AKAP12/Gravin T751A (analogous to T766A mutation in human) in retroviral vector
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 107142 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepBABE
- Backbone size w/o insert (bp) 5198
- Total vector size (bp) 10245
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Vector typeMammalian Expression, Retroviral
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameSSeCKs T751A
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Alt namemurine Gravin/AKAP12
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SpeciesM. musculus (mouse)
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Insert Size (bp)5055
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Mutationchanged threonine 751 to alanine
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Entrez GeneAkap12 (a.k.a. SSeCKS, Srcs5, Tsga12)
- Promoter gag
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer tcgaccccgcctcaatcct (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBABE-SSeCKs T751A was a gift from Heidi Hehnly (Addgene plasmid # 107142 ; http://n2t.net/addgene:107142 ; RRID:Addgene_107142) -
For your References section:
Gravin regulates centrosome function through PLK1. Colicino EG, Garrastegui AM, Freshour J, Santra P, Post DE, Kotula L, Hehnly H. Mol Biol Cell. 2017 Dec 27. pii: mbc.E17-08-0524. doi: 10.1091/mbc.E17-08-0524. 10.1091/mbc.E17-08-0524 PubMed 29282278
Map uploaded by the depositor.
