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1432 pcDNA3 flag MAGI1c
(Plasmid #10714)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 10714 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5446
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MAGI-1
  • Alt name
    MAGI1
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    6000
  • Entrez Gene
    Magi1 (a.k.a. AIP3, BAP1, Baiap1, Gukmi1, MAGI1c, Magi-1, TNRC19, WWP3, mKIAA4129)
  • Tag / Fusion Protein
    • flag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

See author's map for cloning details.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    1432 pcDNA3 flag MAGI1c was a gift from William Sellers (Addgene plasmid # 10714 ; http://n2t.net/addgene:10714 ; RRID:Addgene_10714)

  • For your References section:

    Phosphorylation of the PTEN tail acts as an inhibitory switch by preventing its recruitment into a protein complex. Vazquez F, Grossman SR, Takahashi Y, Rokas MV, Nakamura N, Sellers WR. J Biol Chem. 2001 Dec 28. 276(52):48627-30. 10.1074/jbc.C100556200 PubMed 11707428
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