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PurposeHis tagged human FASN cloned in pcDNA3.1 vector for expression in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 107138 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3.1
- Backbone size w/o insert (bp) 5432
- Total vector size (bp) 12977
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameFatty acid synthase
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Alt nameFASN
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SpeciesH. sapiens (human)
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Insert Size (bp)7545
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GenBank IDNM_004104
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Entrez GeneFASN (a.k.a. FAS, OA-519, SDR27X1)
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Tag
/ Fusion Protein
- His (C terminal on backbone)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3.1_FASN was a gift from Brijesh Kumar Singh (Addgene plasmid # 107138 ; http://n2t.net/addgene:107138 ; RRID:Addgene_107138)