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pET21d-colEdes10/Imdes10
(Plasmid #107118)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 107118 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET21d
  • Backbone size w/o insert (bp) 5440
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    T7 Express lysY/Iq (NEB)
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    colEdes10/Imdes10
  • Species
    Synthetic
  • Promoter T7
  • Tag / Fusion Protein
    • His-tag (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer TAATACGACTCACTATAGGG
  • 3′ sequencing primer GCTAGTTATTGCTCAGCGG
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Two genes in tandem (colE, Im), separated by 2 nucleotides (AA). See partial sequences. AA sequence of colEdes10: MESKRNKPGKATGKGKPVGDKWLDDAGKDSGAPIPDRIADKLRDKEFKNFDDFRKKFWEEVSKDPDLSKQFKGANQDSIKQGQAPFARSKDQVGGRRTFELHHDKPISQDGGVYDMNNIRVTTPKRHIDIHRGK and AA sequence of Imdes10: MELKHSISDYTEAEFLEFVKKIIATNDETQQRALVDEFERLTEHPDGSDLIYYPRDDREDSPEGIVKEIKEWRAANGKSGFKQGLEHHHHHH

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET21d-colEdes10/Imdes10 was a gift from Sarel Fleishman (Addgene plasmid # 107118 ; http://n2t.net/addgene:107118 ; RRID:Addgene_107118)

  • For your References section:

    Ultrahigh specificity in a network of computationally designed protein-interaction pairs. Netzer R, Listov D, Lipsh R, Dym O, Albeck S, Knop O, Kleanthous C, Fleishman SJ. Nat Commun. 2018 Dec 11;9(1):5286. doi: 10.1038/s41467-018-07722-9. 10.1038/s41467-018-07722-9 PubMed 30538236
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