pLJM60 FLAG-RAB35 Q67L
(Plasmid #107105)

• Purpose: Lentiviral vector that codes for FLAG-RAB35 Q67L (human) protein. Puromycin resistance for mammalian selection and ampicillin resistance for growth in Stbl3 cells.
• Depositing Lab: Charles Sawyers
• Publication: Wheeler et al Science. 2015 Oct 9;350(6257):211-7. doi: 10.1126/science.aaa4903. Epub 2015 Sep 3.

Backbone

• Vector backbone: pLJM60
• Backbone manufacturer: Sabatini lab
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: RAB35
• Species: H. sapiens (human)
• Mutation: Q67L
• Entrez Gene: RAB35 (a.k.a. H-ray, RAB1C, RAY)
• Promoter: CMV
• Tag / Fusion Protein:
  • FLAG tag (N terminal on insert)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: pLJM1-3

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Gene insert sequence was synthesized then cloned into the backbone.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry