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pLJM60 FLAG-RAB35 Q67L
(Plasmid #107105)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 107105 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLJM60
  • Backbone manufacturer
    Sabatini lab
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    RAB35
  • Species
    H. sapiens (human)
  • Mutation
    Q67L
  • Entrez Gene
    RAB35 (a.k.a. H-ray, RAB1C, RAY)
  • Promoter CMV
  • Tag / Fusion Protein
    • FLAG tag (N terminal on insert)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Gene insert sequence was synthesized then cloned into the backbone.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLJM60 FLAG-RAB35 Q67L was a gift from Charles Sawyers (Addgene plasmid # 107105 ; http://n2t.net/addgene:107105 ; RRID:Addgene_107105)

  • For your References section:

    Identification of an oncogenic RAB protein. Wheeler DB, Zoncu R, Root DE, Sabatini DM, Sawyers CL. Science. 2015 Oct 9;350(6257):211-7. doi: 10.1126/science.aaa4903. Epub 2015 Sep 3. 10.1126/science.aaa4903 PubMed 26338797
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