pLJM60 FLAG-RAB35 Q67L
(Plasmid
#107105)
-
PurposeLentiviral vector that codes for FLAG-RAB35 Q67L (human) protein. Puromycin resistance for mammalian selection and ampicillin resistance for growth in Stbl3 cells.
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 107105 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepLJM60
-
Backbone manufacturerSabatini lab
-
Vector typeMammalian Expression, Lentiviral
-
Selectable markersPuromycin
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)NEB Stable
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameRAB35
-
SpeciesH. sapiens (human)
-
MutationQ67L
- Promoter CMV
-
Tag
/ Fusion Protein
- FLAG tag (N terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV-F
- 3′ sequencing primer pLJM1-3 (Common Sequencing Primers)
Resource Information
-
A portion of this plasmid was derived from a plasmid made byGene insert sequence was synthesized then cloned into the backbone.
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pLJM60 FLAG-RAB35 Q67L was a gift from Charles Sawyers (Addgene plasmid # 107105 ; http://n2t.net/addgene:107105 ; RRID:Addgene_107105) -
For your References section:
Identification of an oncogenic RAB protein. Wheeler DB, Zoncu R, Root DE, Sabatini DM, Sawyers CL. Science. 2015 Oct 9;350(6257):211-7. doi: 10.1126/science.aaa4903. Epub 2015 Sep 3. 10.1126/science.aaa4903 PubMed 26338797