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Addgene

MSCV mouse Vav1DN IRES GFP
(Plasmid #107092)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 107092 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    MSCV
  • Backbone size w/o insert (bp) 6673
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    GFP

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Vav1 dominant negative cDNA
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    558
  • Mutation
    AA 1-186
  • Entrez Gene
    Vav1 (a.k.a. Vav, vav-T)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (unknown if destroyed)
  • 3′ cloning site XhoI (unknown if destroyed)
  • 5′ sequencing primer unknown
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    The Vav1 cDNA came from Dr. S. Katzav, Hebrew University, Jerusalem, Israel

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MSCV mouse Vav1DN IRES GFP was a gift from Martine Roussel (Addgene plasmid # 107092 ; http://n2t.net/addgene:107092 ; RRID:Addgene_107092)
  • For your References section:

    Cross-species epigenetics identifies a critical role for VAV1 in SHH subgroup medulloblastoma maintenance. Lindsey JC, Kawauchi D, Schwalbe EC, Solecki DJ, Selby MP, McKinnon PJ, Olson JM, Hayden JT, Grundy RG, Ellison DW, Williamson D, Bailey S, Roussel MF, Clifford SC. Oncogene. 2015 Sep 3;34(36):4746-57. doi: 10.1038/onc.2014.405. Epub 2014 Dec 22. 10.1038/onc.2014.405 PubMed 25531316