1319 pcDNA3 flag FKHRL1 AAA
(Plasmid #10709)

• Depositing Lab: William Sellers
• Publication: Ramaswamy et al Cancer Cell. 2002 Jul . 2(1):81-91.

Backbone

• Vector backbone: pcDNA3
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5446
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: FKHRL1 AAA
• Alt name: FOXO3A
• Alt name: FKHRL1
• Species: H. sapiens (human)
• Insert Size (bp): 2000
• Mutation: AAA mutant (see map) - T32A, S253A, S315A
• Entrez Gene: FOXO3 (a.k.a. AF6q21, FKHRL1, FKHRL1P2, FOXO2, FOXO3A)
• Tag / Fusion Protein:
  • flag (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: T7

Resource Information

• Articles Citing this Plasmid:
  • 10 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry