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pET15b-YM1
(Plasmid #107079)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 107079 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET-15b
  • Backbone manufacturer
    Merck Millipore
  • Backbone size w/o insert (bp) 5700
  • Total vector size (bp) 7200
  • Modifications to backbone
    NcoI site was removed and a stop codon was inserted after BamHI site.
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Supplement with 1% glucose is recommended to suppress leaky expression.
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    M. sedula PCNA1 fused to YPet
  • Alt name
    YPet-MsePCNA1
  • Species
    Synthetic
  • Insert Size (bp)
    1600
  • Promoter T7 promoter
  • Tag / Fusion Protein
    • His6 tag (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7 Terminal
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET15b-YM1 was a gift from Teruyuki Nagamune (Addgene plasmid # 107079 ; http://n2t.net/addgene:107079 ; RRID:Addgene_107079)

  • For your References section:

    Three proliferating cell nuclear antigen homologues from Metallosphaera sedula form a head-to-tail heterotrimer. Iwata F, Hirakawa H, Nagamune T. Sci Rep. 2016 May 27;6:26588. doi: 10.1038/srep26588. 10.1038/srep26588 PubMed 27228945
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