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pMD-MsePCNA3
(Plasmid #107075)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 107075 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMD20
  • Backbone manufacturer
    Takara Bio
  • Backbone size w/o insert (bp) 2700
  • Total vector size (bp) 3500
  • Vector type
    Cloning vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    M. sedula PCNA3
  • Alt name
    MsePCNA3
  • Alt name
    Msed_2250
  • Species
    Synthetic; Metallosphaera sedula
  • Insert Size (bp)
    770
  • GenBank ID
    WP_012022175
  • Promoter SP6, lac

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMD-MsePCNA3 was a gift from Teruyuki Nagamune (Addgene plasmid # 107075 ; http://n2t.net/addgene:107075 ; RRID:Addgene_107075)

  • For your References section:

    Three proliferating cell nuclear antigen homologues from Metallosphaera sedula form a head-to-tail heterotrimer. Iwata F, Hirakawa H, Nagamune T. Sci Rep. 2016 May 27;6:26588. doi: 10.1038/srep26588. 10.1038/srep26588 PubMed 27228945
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