1264 pBS RSV Gal4 FKHR
(Plasmid
#10707)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 10707 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBS RSV Gal4
- Backbone size w/o insert (bp) 3000
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameFKHR
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Alt nameFOXO1A
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SpeciesH. sapiens (human)
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Insert Size (bp)2000
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Mutation(the Gal4 DBD is aa 1-147)
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Entrez GeneFOXO1 (a.k.a. FKH1, FKHR, FOXO1A)
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Tag
/ Fusion Protein
- Gal4 DBD (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer n/a
- 3′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
1264 pBS RSV Gal4 FKHR was a gift from William Sellers (Addgene plasmid # 10707 ; http://n2t.net/addgene:10707 ; RRID:Addgene_10707)
Map uploaded by the depositor.