pGC1::YC3.60
(Plasmid
#107061)
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PurposeExpressing Ca sensor yellow cameloen 3.6 under guard cell specific promoter
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 107061 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepGreenII 0179
- Total vector size (bp) 9069
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Vector typePlant Expression
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameYC3.6
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Alt nameyellow cameleon 3.6
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SpeciesSynthetic
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Insert Size (bp)1962
- Promoter GC1 (At1g22690)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer gtaaaacgacggccagt
- 3′ sequencing primer gtcatagctgtttcctg (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGC1::YC3.60 was a gift from Julian Schroeder (Addgene plasmid # 107061 ; http://n2t.net/addgene:107061 ; RRID:Addgene_107061) -
For your References section:
Isolation of a strong Arabidopsis guard cell promoter and its potential as a research tool. Yang Y, Costa A, Leonhardt N, Siegel RS, Schroeder JI. Plant Methods. 2008 Feb 19;4:6. doi: 10.1186/1746-4811-4-6. 10.1186/1746-4811-4-6 PubMed 18284694