pTRIPZ Myc2-mouse TTP aa S52 S178 MUT
(Plasmid #107001)

• Purpose: Expresses epitope-tagged double-mutant murine TTP
• Depositing Lab: Julian Downward
• Publication: Coelho et al Immunity. 2017 Dec 19;47(6):1083-1099.e6. doi: 10.1016/j.immuni.2017.11.016. Epub 2017 Dec 12.

Backbone

• Vector backbone: pTRIPZ
• Backbone manufacturer: Thermo Scientific
• Modifications to backbone: An Age1/Mlu1 digest deletes the TurboRFP and shRNA sites of the parental plasmid and inserts are pasted in downstream of the tet operator and just upstream of the UbC promoter, which is driving the tet system
• Vector type: Mammalian Expression
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: tristetraprolin
• Alt name: TTP, ZFP36
• Species: M. musculus (mouse)
• Insert Size (bp): 1724
• Mutation: Changed Ser52 to Ala and Ser178 to Ala
• Entrez Gene: Zfp36 (a.k.a. Gos24, Nup475, TIS11D, TISII, Tis11, Ttp, Zfp-36)
• Tag / Fusion Protein:
  • Double Myc peptide epitope tag (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AgeI (unknown if destroyed)
• 3′ cloning site: MluI (unknown if destroyed)
• 5′ sequencing primer: pCEP fwd
• 3′ sequencing primer: must sequence out of the insert

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note- Addgene's quality control found one “g” nucleotide missing from the insert sequence. The depositor noted this "g" should make no difference to the function of plasmid, since it is located within an intron and will be spliced out.