pDest-Sumo
(Plasmid #106980)

• Purpose: (Empty Backbone) LR reaction to create expression plasmid. For prokaryotic expression of target protein as N-terminal fusion form with Sumo tag
• Depositing Lab: Han Choe
• Publication: Nguyen et al Sci Rep. 2017 Nov 23;7(1):16139. doi: 10.1038/s41598-017-16167-x.

Backbone

• Vector backbone: pDEST-0GWA
• Backbone size (bp): 7432
• Modifications to backbone: insert His8-Sumo
• Vector type: Bacterial Expression
• Promoter: T7
• Tag / Fusion Protein:
  • His8-Sumo

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol and Ampicillin, 25 & 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): ccdB Survival
• Growth instructions: Must be grown in a strain resistant to ccdB
• Copy number: Unknown

Cloning Information

• Cloning method: Gateway Cloning

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry