pEGFP-N1 Full length Importin beta
(Plasmid #106941)

• Purpose: Transient expression of GFP-tagged Importin beta in mammalian cells (CMV promoter)
• Depositing Lab: Patrizia Lavia
• Publication: Ciciarello et al J Cell Sci. 2004 Dec 15. 117(Pt 26):6511-22.

Backbone

• Vector backbone: pEGFP-N1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4700
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: human Importin beta-1 (KPNB1)
• Alt name: karyopherin beta-1 (KPN1)
• Species: H. sapiens (human)
• Insert Size (bp): 2631
• Entrez Gene: KPNB1 (a.k.a. IMB1, IPO1, IPOB, Impnb, NTF97)
• Promoter: CMV promoter
• Tag / Fusion Protein:
  • GFP (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SacI (unknown if destroyed)
• 3′ cloning site: BamHI (unknown if destroyed)

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid was created by Antonella Palena in the Lavia Lab.