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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 10694 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5446
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameFKHR deltaDB
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Alt nameFOXO1A
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Alt nameFKHR
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SpeciesH. sapiens (human)
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Insert Size (bp)1900
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Mutationdeletion of amino acids 208-220
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Entrez GeneFOXO1 (a.k.a. FKH1, FKHR, FOXO1A)
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Tag
/ Fusion Protein
- flag (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer T7 (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
1354 pcDNA3 flag FKHR deltaDB was a gift from William Sellers (Addgene plasmid # 10694 ; http://n2t.net/addgene:10694 ; RRID:Addgene_10694)
Map uploaded by the depositor.
![](https://media.addgene.org/data/easy-thumbnails/data/80/30/32761054-af61-11e0-90fe-003048dd6500.jpeg.940x940_q85_autocrop.png)