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Addgene

pX-RCC1-HA
(Plasmid #106938)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 106938 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBluescript-derived pX expression vector
  • Backbone manufacturer
    Pidder Jansen-Durr lab; pX derives from commercial pBluescript
  • Backbone size w/o insert (bp) 3985
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    human RCC1-HA
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1293
  • Entrez Gene
    RCC1 (a.k.a. CHC1, RCC1-I)
  • Promoter CMV promoter
  • Tag / Fusion Protein
    • HA (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (unknown if destroyed)
  • 3′ cloning site SalI (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This plasmid was created by Antonella Palena in the Lavia Lab.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pX-RCC1-HA was a gift from Patrizia Lavia (Addgene plasmid # 106938 ; http://n2t.net/addgene:106938 ; RRID:Addgene_106938)

  • For your References section:

    E1A deregulates the centrosome cycle in a Ran GTPase-dependent manner. DeLuca A, Mangiacasale R, Severino A, Malquori L, Baldi A, Palena A, Mileo AM, Lavia P, Paggi MG. Cancer Res. 2003 Mar 15;63(6):1430-7. PubMed 12649209
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