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PurposeExpress ERK-SPARK, an activity reporter of ERK in mammalian cells.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 106921 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA3
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameERK-SPARK
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3-ERK-SPARK was a gift from Xiaokun Shu (Addgene plasmid # 106921 ; http://n2t.net/addgene:106921 ; RRID:Addgene_106921) -
For your References section:
Visualizing Dynamics of Cell Signaling In Vivo with a Phase Separation-Based Kinase Reporter. Zhang Q, Huang H, Lu Q, Wu R, Chung CI, Zhang SQ, Torra J, Schepis A, Coughlin SR, Kornberg TB, Shu X. Mol Cell. 2018 Jan 3. pii: S1097-2765(17)30936-X. doi: 10.1016/j.molcel.2017.12.008. 10.1016/j.molcel.2017.12.008 PubMed 29307513
