(PM-S608D)FLAG-Cep215-FL
(Plasmid
#106909)
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PurposeExpresses murine phospho-mimetic CEP215 at S608
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 106909 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3
- Backbone size w/o insert (bp) 5400
- Total vector size (bp) 10870
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameCEP215
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Alt nameCDK5RAP2
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SpeciesM. musculus (mouse)
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Insert Size (bp)5470
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Mutationchanged Serine-608 to Aspartic Acid
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Entrez GeneCdk5rap2 (a.k.a. 2900018K03Rik, an, mKIAA1633)
- Promoter CMV
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Tag
/ Fusion Protein
- FLAG (N terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer T7 promoter
- 3′ sequencing primer SP6 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
(PM-S608D)FLAG-Cep215-FL was a gift from Heidi Hehnly (Addgene plasmid # 106909 ; http://n2t.net/addgene:106909 ; RRID:Addgene_106909)