PLK1-cjun-FRET-PACT biosensor
(Plasmid
#106907)
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Purposecentrosome-localized PLK1 FRET-activity biosensor (through PACT domain with 10aa linker)
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 106907 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA3.1
- Backbone size w/o insert (bp) 5331
- Total vector size (bp) 8078
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namePLK1-cjun-FRET-PACT biosensor with linker
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SpeciesH. sapiens (human)
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Insert Size (bp)2739
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Entrez GenePLK1 (a.k.a. PLK, STPK13)
- Promoter CMV
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Tag
/ Fusion Protein
- PACT domain (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer T7 promoter
- 3′ sequencing primer SP6 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
PLK1-cjun-FRET-PACT biosensor was a gift from Heidi Hehnly (Addgene plasmid # 106907 ; http://n2t.net/addgene:106907 ; RRID:Addgene_106907) -
For your References section:
Gravin regulates centrosome function through PLK1. Colicino EG, Garrastegui AM, Freshour J, Santra P, Post DE, Kotula L, Hehnly H. Mol Biol Cell. 2017 Dec 27. pii: mbc.E17-08-0524. doi: 10.1091/mbc.E17-08-0524. 10.1091/mbc.E17-08-0524 PubMed 29282278
Map uploaded by the depositor.
