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(PD-S608A)FLAG-Cep215-FL
(Plasmid #106905)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 106905 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3
  • Backbone size w/o insert (bp) 5400
  • Total vector size (bp) 10870
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    CEP215
  • Alt name
    CDK5RAP2
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    5470
  • Mutation
    changed Serine-608 to Alanine
  • Entrez Gene
    Cdk5rap2 (a.k.a. 2900018K03Rik, an, mKIAA1633)
  • Promoter CMV
  • Tag / Fusion Protein
    • FLAG (N terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    (PD-S608A)FLAG-Cep215-FL was a gift from Heidi Hehnly (Addgene plasmid # 106905 ; http://n2t.net/addgene:106905 ; RRID:Addgene_106905)

  • For your References section:

    Gravin regulates centrosome function through PLK1. Colicino EG, Garrastegui AM, Freshour J, Santra P, Post DE, Kotula L, Hehnly H. Mol Biol Cell. 2017 Dec 27. pii: mbc.E17-08-0524. doi: 10.1091/mbc.E17-08-0524. 10.1091/mbc.E17-08-0524 PubMed 29282278
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