pFC902
(Plasmid #106904)

• Purpose: The vector has a U3 promoter and terminator controlled gene encoding a transcript containing the sgRNA flanked by a tRNA-Gly repeat; and a gene encoding cas9 controlled by Ptef1 and Ttef1
• Depositing Lab: Uffe Mortensen
• Publication: Nodvig et al Fungal Genet Biol. 2018 Jan 8. pii: S1087-1845(18)30004-5. doi: 10.1016/j.fgb.2018.01.004.

Backbone

• Vector backbone: custom
• Total vector size (bp): 16702
• Modifications to backbone: AMA1 element
• Vector type: Bacterial Expression, CRISPR ; Fungal expression
• Selectable markers: pyrG

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: cas9
• Species: Synthetic; synthetic gene, codon optimized for A. niger
• Insert Size (bp): 4131
• GenBank ID: KT031983
• Tag / Fusion Protein:
  • SV40 NLS (C terminal on insert)

Gene/Insert 2

• Gene/Insert name: pyrG
• Species: Aspergillus fumigatus
• Insert Size (bp): 905

Gene/Insert 3

• Gene/Insert name: U3 promoter and terminator controlled gene encoding a transcript containing the sgRNA flanked by a tRNA gly repeat
• Insert Size (bp): 1845

Resource Information

• Supplemental Documents:
  • pCRISPR1-t1p.gbk
• A portion of this plasmid was derived from a plasmid made by: The cas9 gene contained in the vector is a custom made version that we bought from GenScript. pFC902 contains the same pyrG marker as in pFC330 (Plasmid 87842) and the same AMA1 sequence as in pFC330 (Plasmid 87842).
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry