pQE30del
(Plasmid
#106902)
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Purpose(Empty Backbone) pQE30del is conditionally replicating empty vector. Replication of the plasmid is inhibited in presense of IPTG or lactose. pQE30del can be used for construction of any E. coli helper plasmids.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 106902 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepQE30
- Backbone size (bp) 3461
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Growth instructionspQE30del can stably replicate only in presence of lacIQ allele
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Copy numberHigh Copy
Cloning Information
- Cloning method Restriction Enzyme
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pQE30del was a gift from Sergey Sineoky (Addgene plasmid # 106902 ; http://n2t.net/addgene:106902 ; RRID:Addgene_106902) -
For your References section:
Development of new versatile plasmid-based systems for lambdaRed-mediated Escherichia coli genome engineering. Bubnov DM, Yuzbashev TV, Vybornaya TV, Netrusov AI, Sineoky SP. J Microbiol Methods. 2018 Jun 7. pii: S0167-7012(18)30368-3. doi: 10.1016/j.mimet.2018.06.001. 10.1016/j.mimet.2018.06.001 PubMed 29885886
Map uploaded by the depositor.
