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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 10689 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepBABE-zeo
- Backbone size w/o insert (bp) 4888
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Vector typeMammalian Expression, Retroviral
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Selectable markersZeocin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameprotein phosphatase 2, catalytic subunit, alpha isozyme
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SpeciesH. sapiens (human)
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Insert Size (bp)1770
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GenBank IDNM_002715
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Entrez GenePPP2CA (a.k.a. HJS3, NEDLBA, PP2Ac, PP2CA, PP2Calpha, RP-C)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer pBABE 5' (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBABE zeo PPP2CA Wt was a gift from William Hahn (Addgene plasmid # 10689 ; http://n2t.net/addgene:10689 ; RRID:Addgene_10689) -
For your References section:
Identification of specific PP2A complexes involved in human cell transformation. Chen W, Possemato R, Campbell KT, Plattner CA, Pallas DC, Hahn WC. Cancer Cell. 2004 Feb . 5(2):127-36. 10.1016/S1535-6108(04)00026-1 PubMed 14998489
